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Hormones and Hemodynamics in Pregnancy


1 Division of Renal Diseases and Hypertension, University of Colorado Denver, Denver, Colorado, USA
*Corresponding author: Robert W. Schrier, Department of Medicine, University of Colorado, 12700 East 19th Avenue C281, Aurora, CO 80045, USA. Tel: +1-3037244837, Fax: +1-3037244868, E-mail: robert.schrier@ucdenver.edu.
International Journal of Endocrinology and Metabolism. 2014 April; 12(2): e14098 , DOI: 10.5812/ijem.14098
Article Type: Review Article; Received: Sep 18, 2013; Revised: Oct 15, 2013; Accepted: Dec 10, 2013; epub: Apr 1, 2014; ppub: Apr 2014

Abstract


Context:: Normal pregnancy is associated with sodium and water retention, which results in plasma volume expansion prior to placental implantation. The explanation offered for these events is that pregnancy ‘resets’ both volume and osmoreceptors.

Evidence Acquisition:: The mechanisms for such an enigmatic ‘resetting’ in pregnancy have not previously been explained. However, recent human pregnancy studies have demonstrated that the earliest hemodynamic change in pregnancy is primary systemic arterial vasodilation. This arterial underfilling is associated with a secondary increase in cardiac output and activation of the neurohumoral axis, including stimulation of the renin-angiotensin-aldosterone, sympathetic, and non-osmotic vasopressin systems. Resistance to the pressor effects of angiotensin and sympathetic stimulation in pregnancy is compatible with an increase in endothelial nitric oxide synthase activity.

Results:: In contrast to the sodium and water retention which occur secondary to the primary arterial vasodilation in cirrhosis, glomerular filtration and renal blood flow are significantly increased in normal pregnancy. A possible explanation for this difference in arterial vasodilation states is that relaxin, an arterial vasodilator which increases during pregnancy, has a potent effect on both systemic and renal circulation. Endothelial damage in pregnancy is pivotal in the pathogenesis of preeclampsia in pregnancy.

Conclusions:: Against a background of the primary arterial vasodilation hypothesis, it is obvious that reversal of the systemic vasodilatation in pregnancy, without subsequent activation of the renin-angiotensin-aldosterone system (78), will evoke a reversal of all the links in the chain of events in normal pregnancy adaptation, thus, it may cause preeclampsia. Namely, a decrease of renal vasodilation will decrease glomerular filtration rate.

Keywords: Pregnancy; Arterial; Osmolar Concentration; Relaxin; Nitric Oxide; Pre-Eclampsia, Endothelium

1. Context


Normal human pregnancy is characterized by physiologic changes in neurohumoral status, systemic and renal hemodynamics, as well as changes in sodium and water balance. Specifically, there is a decrease in mean arterial pressure (MAP) (1) and plasma osmolality (1-3) and an increase in total body electrolytes and water (3, 4). Decreased blood pressure occurs in spite of increased activation of the renin-angiotensin-aldosterone system (RAAS) (5, 6). The causes and sequence of all these phenomena have not been completely defined. Nevertheless, primary systemic vasodilatation is a pivotal feature of pregnancy. Studies in animals (7) and humans (1) have shown that hemodynamic changes in pregnancy occur early, prior to complete placentation. These gestational alterations are qualitatively comparable to the luteal phase of the menstrual cycle, but to a lesser degree (8, 9). Similarly, exaggerated changes occur during ovarian hyperstimulation (10). Changes in sodium and water status are the other remarkable adaptations of normal pregnancy in humans. They are characterized by increases in total body water by 8 L (4), and total body sodium by 1000 mEq (11), as plasma osmolality falls (1-3, 12) by 10 mosmol/kg below nonpregnant levels (1, 13).

2. Evidence Acquisition


2.1. Pregnancy is a unique state of arterial underfilling

The primary arterial vasodilation hypothesis of sodium and water retention explains many of these pregnancy changes (14). Thus, the unifying hypothesis of body fluid volume regulation can apply to pregnancy. A normal kidney regulates sodium and water excretion, not primarily in response to total blood volume, but rather by the effective arterial blood volume (EABV) (15). A diminished cardiac output or primary arterial vasodilation triggers a decrease in EABV. This leads to the activation of the RAAS and sympathetic nervous systems and non-osmotic release of arginine vasopressin (AVP), with resultant renal sodium and water retention to compensate for the decrease in EABV. The stretch arterial baroreceptors can be unloaded with stimulation of the neurohumoral axis and cardiac output, even in the presence of increased total blood volume, since an estimated 85% of circulatory blood resides in the venous circulation. The remaining 15% of blood is located in arterial circulation and primarily determines renal sodium and water regulation. Since pregnancy is a unique state of arterial under-filling, its physiology can be understood in the light of other states involving arterial underfilling, such as; hepatorenal or cardiorenal syndromes (Figure 1) (14). However, it should be emphasized that pregnancy has several unique features, namely escape from the sodium-retaining effects of aldosterone, increase in glomerular filtration rate (GFR), and renal blood flow (RBF), in spite of a decrement in systemic vascular resistance (SVR). The initial systemic and renal hemodynamic and neurohumoral changes that occur in early human pregnancy have only now been well defined (Figures 2, 3) (1). SVR decreases significantly by week 6 of gestation and causes a fall in MAP, which leads to a compensatory increase in cardiac output (1, 16). Renal vasodilation occurs simultaneously with systemic vasodilatation and it is also associated with a 30-50% increase in renal blood flow and GFR (1, 17). These increases in renal hemodynamics distinguish pregnancy from other states of systemic vasodilation in which renal vasoconstriction occurs, as a rise in cardiac output is inadequate to maintain MAP. The RAAS is stimulated and circulating levels of renin, angiotensin II (ANG II) and aldosterone rise in early pregnancy (5, 6, 18). This hormonal activation causes sodium and water retention, which leads to the expansion of total plasma volume (1). As might be expected, an increment in atrial natriuretic peptide (ANP) follows volume expansion in pregnancy (1). However an increase in GFR (19) and cardiac output (20) precedes blood volume expansion, and therefore it is related to primary arterial vasodilation of the renal and systemic circulation. Studies of pregnant baboons have also demonstrated that the blood volume expansion occurs after a decrease in SVR and stimulation of the RAAS axis (20). If the blood volume expansion were primary, rather than secondary to the arterial vasodilatation, suppression, not stimulation, of the RAAS would be expected. Unlike other states with primary vasodilatation, escape from the sodium-retaining effects of aldosterone occurs in pregnancy (17).

Figure 1.
Body Fluid Regulation in Health and Disease: A Unifying Hypothesis., Renin-Angiotensin-Aldosterone System (RAAS); Sympathetic Nervous System (SNS). Reproduced with Permission from Ref. 19.
Figure 2.
Ten Women Studied in the Mid-Follicular Phase of the Menstrual Cycle and Weeks 6, 8, 10, 12, 24 and 36 Gestation. Reproduced with Permission from Ref. 1.
Figure 3.
Ten Women Were Studied in the Mid-Follicular Phase of the Menstrual Cycle and Weeks 6, 8, 10, 12, 24 and 36 Gestation Reproduced With Permission From Ref. 1

Besides the increase in progesterone (14), the rise in GFR in pregnancy increases distal sodium delivery, thereby, allowing for further escape from the sodium retaining effect of aldosterone (21). There is also the involvement of the distal tubule in aldosterone sodium escape (21). The volume expansion secondary to aldosterone increases ANP which inhibits sodium reabsorption in the distal tubule. However, angiotensin-mediated upregulation of the epithelial sodium channel (ENaC) and phosphodiesterases in the distal tubule opposes the effect of ANP (22). With respect to the effect of aldosterone to increase potassium secretion in the distal tubule, the increased distal sodium delivery with aldosterone-mediated volume expansion will enhance this effect on potassium excretion (23). A genetic variant of ENaC has been found to be associated with preeclampsia (24).

2.2. Volume Expansion

As mentioned previously, water and sodium-retaining hormones are stimulated in pregnancy leading to renal sodium and water retention. Resultant plasma volume expansion compensates for arterial underfilling, which occurs with primary arterial vasodilation. This volume expansion is extremely important for normal fetal development in either animal or human pregnancy. Indeed, water deprivation and salt restriction causes a reduction of fetal weight in rats (25, 26). In humans, idiopathic fetal growth restriction was also associated with reduced plasma volume (27).

2.3. Osmoregulation

Another prominent feature of healthy pregnancy is the occurrence of hypoosmolality. This water retention and decreased body tonicity in pregnancy has been a focus of previous investigations (13). Negative free water clearance in healthy pregnant women indicates that free water is steadily retained during pregnancy (28). Rats are an excellent animal model to study osmoregulation in pregnancy, because their blood contains no detectable vasopressinase (29). In humans, vasopressinase is produced by the placenta and it inactivates AVP by clipping the hormone’s first amino acid (30). Thus, the measurement of plasma vasopressin in human pregnancy necessitates the inhibition of vasopressinase (29). An animal study has shown that the threshold for AVP secretion decreases in rat pregnancy, even though urinary diluting and concentrating ability is preserved (31). Studies in human pregnancy have shown comparable results (32, 33). This led to the assumption that in pregnancy, osmoregulation is ‘reset’ at a lower osmolality around a new steady state (31). The threshold for thirst was also found to be ‘reset’ at a lower osmolality plasma level, thereby facilitating water retention (31, 33). The primary arterial vasodilation hypothesis of sodium and water retention in pregnancy is compatible with this ‘reset’ osmostat as mediated by nonosmotic AVP stimulation (14). The finding that plasma AVP is not suppressed in pregnancy in the presence of hyponatremia and hypotonicity supports this hypothesis (31, 33, 34). The urinary excretion of AVP is also increased in pregnancy and relates inversely to urine flow rate and free water clearance. This further supports the role of AVP in hyponatremia in pregnancy (28).

Xu et al. were able to show not only increased plasma AVP, but also an increase in hypothalamic AVP mRNA in pregnant rats in the presence of hypoosmolality (Figure 4) (35). Vasopressin-mediated water reabsorption in the collecting duct occurs via aquaporin 2 (AQP2) water channels by the activation of vasopressin 2 receptors (36). Since upregulation of AQP2 was observed in other states of arterial underfilling, namely cirrhosis (37) and cardiac failure (38), it was extremely important to measure AQP2 expression in pregnancy. A significant increase in AQP2 was observed by day 7 of gestation (Figure 5) (34). Administration of V2 receptor antagonist led to diuresis, urinary dilution and decrease in the AQP2 protein to nonpregnant levels. The unexpected persistence of hypoosmolality, despite the V2 receptor blockade, can be explained by polydipsia considering the ‘reset’ of the thirst threshold. This confirmed the role of V2 receptors in the upregulation of AQP2 during pregnancy. Similar results were demonstrated in human pregnancy (39).

Figure 4.
Hypoosmolality in Pregnancy (A) Associated with Increased Plasma Arginine Vasopressin (AVP) (B) and a Rise in Hypothalamic AVP (C).
Figure 5.
Chronic Nitric Oxide Synthase (NOS) Inhibition Returns Cardiac Output (CO) and Systemic Vascular Resistance (SVR) in Day 14 Pregnant Rats to Nonpregnant Levels.
2.4. Potential Mediation of Vasodilation of Pregnancy

Relaxin, the peptide hormone produced by the corpus luteum under the stimulation of human chorionic gonadotropin (HCG), has been suggested to play an important role in the regulation of the hemodynamics and water metabolism in pregnancy. Chronic administration of a recombinant human relaxin (rhRLX) to nonpregnant animals increased cardiac output and arterial compliance (40), reduced SVR (40, 41), and decreased renal vascular resistance (RVR) (42), in association with increased RBF and GFR. In human studies, acute infusion of rhRLX increased the cardiac index, and reduced SVR, serum creatinine (43) and RBF (44). Chronic administration raised creatinine clearance and reduced systolic blood pressure (45, 46). The Conrad group proposed the working model of the relaxin sustained-vasodilatory response, which develops in response to prolonged (hours or days) relaxin exposure (47). It was hypothesized that vascular endothelial growth factor (VEGF) initiates the gelatinase-ETB-receptor-NO-pathway in endothelium after relaxin activates the relaxin/insulin-like family (RXFP1) in vascular smooth muscles (47). Relaxin, therefore, stimulates the formation of endothelin through an alternative pathway with the help of matrix metalloproteinase gelatinases (45, 47-49), and endothelin in turn mediates vasodilation via nitric oxide (NO) synthesis. Increased NO levels in vascular beds (50), raised levels of plasma and urinary NO metabolites (51), as well as upregulated endothelial NO-synthase (NOS) in the arteries of pregnant rats (35, 52), and supported NO as a mediator of arterial vasodilation. Moreover, both the renal and systemic vasodilation response to pregnancy was prevented by NOS-inhibition (53); however, this occurred in association with significant hypertension, ie. potential model of preeclampsia. In pregnant rats, NOS-inhibition in a smaller dose - which did not induce hypertension - also reversed the systemic vasodilation and returned renal hemodynamics to nonpregnant levels. This supported a role for NO as a mediator of glomerular hyperfiltration and renal vasodilation in pregnancy (54). Relaxin binding sites are present in the brain (55) and intracerebroventricular administration of relaxin leads to activation of AVP containing neurons (56), increased thirst response (57), and AVP release (58). However, these studies were conducted only in animals (41, 59, 60) and results may differ significantly from human studies. In women undergoing in vitro fertilization (61, 62), and thus who lack relaxin, plasma osmolality nevertheless decreased, suggesting that primary vasodilation operates. Most relaxin-sensitive brain neurons are sensitive to ANG-II (63) and ANG-II (64, 65) stimulated relaxin-induced thirst. Thus, relaxin may be involved in the stimulation of thirst in pregnancy.

The vascular refractoriness to vasoconstrictors, including angiotensin II and norepinephrine, contributes to profound vasodilation in healthy pregnancy (66). Vascular insensitivity to ANG II during normal pregnancy may be the result of a decrease in ANG II receptors (67), or increased progesterone and prostacyclins (66), or relaxin’s effect (60). Notably, a similar refractoriness to the pressor effects of injected ANG II is exhibited by patients with cirrhosis and ascites, which is another state of vasodilation (68).

2.5. Implications for Preeclampsia

Understanding the physiology of healthy pregnancy will provide insights into the pathology of pregnancy, namely preeclampsia. This unique disorder of human pregnancy remains the leading cause of infant and maternal morbidity and mortality worldwide (69). A decrease in systemic and renal vascular resistance is the main physiologic adaptation of normal pregnancy, but this is not the case in preeclampsia. It is now clear that the hemodynamics of untreated preeclampsia is characterized by increased SVR (70, 71).

3. Results


Most likely, this loss of systemic vasodilation in preeclampsia is mediated by endothelial damage. Novel insights explain endothelial damage in preeclampsia by the release of antiangiogenic factors from an ischemic placenta. Increased placental production of soluble fms-like tyrosine kinase 1 (Flt1), an alternative splice variant of the VEGF receptor fms-like tyrosine kinase 1, might oppose physiologic vasorelaxation by blocking vasodilation induced by VEGF and placental growth factor (PGF) (72). A placenta-derived endoglin (Eng) - a soluble coreceptor for transforming growth factor (TGF)-β1 isoform - which is elevated in preeclamptic individuals, blocks TGF-β1-mediated activation of eNOS, and thus NO-dependant vasodilation (73).

Loss of vascular refractoriness to vasoconstrictors, which is characteristic of normal pregnancy (66), contributes to pronounced vasoconstriction in preeclampsia. In particular, Gant showed increased vascular sensitivity to ANG II in preeclampsia (74). However, the exact mechanism underlying this phenomenon is still unclear. Identified heterodimerization of AT1-receptors (receptors for ANG II) and B2-receptors (receptors for bradykinin) mediates, at least in part, an increased responsiveness to ANG II in preeclamptic hypertensive women (75). Stimulatory IgG autoantibodies against AT1 receptors found in preeclamptic women may also provoke this exaggerated pressor response to ANG II (76). Plasma ANG (1-7) which produces depressor, vasodilatory, and antihypertensive effects, is increased by 51% in normal pregnancy; in contrary, in preeclamptic subjects plasma ANG is decreased significantly and this might contribute to ANG II sensitivity as well (77).

4. Conclusions


Against the background of the Primary Arterial Vasodilation hypothesis, it is obvious that a reversal of systemic vasodilatation in pregnancy, without subsequent activation of the RAAS (78), will evoke a reversal of all links in the chain of events in normal pregnancy adaptation, therefore may cause preeclampsia. That is to say, a decrease in renal vasodilation will decrease GFR. A decrease in GFR will impair the aldosterone escape mechanism with a subsequent increase in sodium retention. Increased sodium retention will increase water retention and vascular sensitivity to vasoconstrictors, thus causing hypertension and edema. Increased ANG sensitivity through hemodynamic (79) and non-hemodynamic (80) mechanisms will enhance glomerular permeability to macromolecules and induce proteinuria. Thus pathological reversal of systemic vasorelaxation abrogates hemodynamic and homeostatic changes in pregnancy and leads to hypertension, edema and proteinuria, the diagnostic triad of preeclampsia.

Acknowledgments

Dr. Tkachenko acknowledges support from the International Society of Nephrology.

Footnotes

Implication for health policy makers/practice/research/medical education: This article reviews the normal physiology of pregnancy relative to early systemic arterial vasodilation. Previous studies about pregnancy have been performed in the rat; therefore studies on the normal physiology of human pregnancy are pivotal in understanding the pathophysiology of preeclampsia/eclampsia. This has many implications for health policy, education, and research since preeclampsia/eclampsia is the major cause of morbidity and mortality in pregnancy for both mother and child. There is also a need to understand the causes and potential prevention methods of endothelial damage in preeclampsia/eclampsia in order to attenuate such complications.
Authors’ Contribution: Drs. Tkachenko and Schrier were responsible for the design of the article; collection, management, and analysis of the data; and preparation, review, and approval of the manuscript. Dr. Shchekochikhin contributed in the design, preparation, and review of the manuscript.
Financial Disclosure: The authors confirm no conflict of interest in this article content.
Funding/Support: The funding organization is a public institution with no role in the design and conduct of the study; collection, management, and analysis of the data; or preparation, review, and approval of the manuscript. Dr. Tkachenko acknowledges support from the International Society of Nephrology.

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Figure 1.

Body Fluid Regulation in Health and Disease: A Unifying Hypothesis., Renin-Angiotensin-Aldosterone System (RAAS); Sympathetic Nervous System (SNS). Reproduced with Permission from Ref. 19.

Figure 2.

Ten Women Studied in the Mid-Follicular Phase of the Menstrual Cycle and Weeks 6, 8, 10, 12, 24 and 36 Gestation. Reproduced with Permission from Ref. 1.
A. Systemic hemodynamic changes throughout early human pregnancy. Mean arterial pressure (MAP) decreased and cardiac output (CO) increased significantly by week 6 gestation in association with a decrease in systemic vascular resistance (SVR). *P < 0.05, **P < 0.001. Figure 2B . Plasma volume (PV), blood volume (BV), and red cell mass (RCM) determinations in early pregnancy. Plasma and blood volume increased significantly by week 6 gestation. Red cell mass remained unchanged throughout pregnancy. *P < 0.05, **P < 0.0001.

Figure 3.

Ten Women Were Studied in the Mid-Follicular Phase of the Menstrual Cycle and Weeks 6, 8, 10, 12, 24 and 36 Gestation Reproduced With Permission From Ref. 1
A. Renal hemodynamic changes throughout early human pregnancy. Renal plasma flow and glomerular filtration rates increased significantly in association with a renal vascular resistance by week 6 gestation. Twenty-four-hour urinary creatinine excretion remained unchanged throughout gestation. Abbreviations: CIn, inulin clearance; CPAH, para-aminohippurate clearance; RVR, renal vascular resistance; UCrV, urinary creatinine excretion. *P < 0.05, **P < 0.001. B. Vasopressor hormone profiles throughout early human pregnancy. Plasma renin activity (PRA) and aldosterone (Aldo) levels increased by week 6 gestation. Norepinephrine (Norepi) concentrations did not change throughout gestation. Atrial natriuretic peptide (ANP) concentrations increased significantly by week 12 gestation (**P < 0.001.).

Figure 4.

Hypoosmolality in Pregnancy (A) Associated with Increased Plasma Arginine Vasopressin (AVP) (B) and a Rise in Hypothalamic AVP (C).

Figure 5.

Chronic Nitric Oxide Synthase (NOS) Inhibition Returns Cardiac Output (CO) and Systemic Vascular Resistance (SVR) in Day 14 Pregnant Rats to Nonpregnant Levels.
Values are means + /- SE for CO (A), and calculated SVR (A). *P < 0.05 vs. NP-control (CTRL) and vs. pregnant (Preg) + nitro-L-arginine methyl ester (NAME). Chronic NOS inhibition reverses glomerular hyperfiltration and renal vasodilation in day 14 pregnant rats. B: glomerular filtration rate (GFR); renal plasma flow (RPF). *P < 0.01 vs. NP-CTRL and vs. Preg+NAME; **P < 0.05 vs. NP-CTRL. Reproduced with permission from Ref. 50.