International Journal of Endocrinology and Metabolism

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The Effect of Leptin and Adiponectin on KiSS-1 and KissR mRNA Expression in Rat Islets of Langerhans and CRI-D2 Cell Line

Mandana Mahmoodzadeh Sagheb 1 , * , Negar Azarpira 2 and Ramin Yaghobi 2
Authors Information
1 Department of Biology, Kazeroon Branch, Islamic Azad University, Kazeroon, IR Iran
2 Transplant Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
Article information
  • International Journal of Endocrinology and Metabolism: April 01, 2014, 12 (2); e15297
  • Published Online: April 1, 2014
  • Article Type: Research Article
  • Received: October 17, 2013
  • Revised: January 25, 2014
  • Accepted: February 25, 2014
  • DOI: 10.5812/ijem.15297

To Cite: Mahmoodzadeh Sagheb M, Azarpira N, Yaghobi R. The Effect of Leptin and Adiponectin on KiSS-1 and KissR mRNA Expression in Rat Islets of Langerhans and CRI-D2 Cell Line, Int J Endocrinol Metab. 2014 ; 12(2):e15297. doi: 10.5812/ijem.15297.

Copyright © 2014, Research Institute For Endocrine Sciences and Iran Endocrine Society. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License ( which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
1. Background
2. Objectives
3. Materials and Methods
4. Results
5. Discussion
  • 1. Klaus S. Adipose tissue as a regulator of energy balance. Curr Drug Targets. 2004; 5(3): 241-50[PubMed]
  • 2. Ahima RS, Lazar MA. Adipokines and the peripheral and neural control of energy balance. Mol Endocrinol. 2008; 22(5): 1023-31[DOI][PubMed]
  • 3. Bandaru P, Shankar A. Association between plasma leptin levels and diabetes mellitus. Metab Syndr Relat Disord. 2011; 9(1): 19-23[DOI][PubMed]
  • 4. Kiess W, Anil M, Blum WF, Englaro P, Juul A, Attanasio A, et al. Serum leptin levels in children and adolescents with insulin-dependent diabetes mellitus in relation to metabolic control and body mass index. Eur J Endocrinol. 1998; 138(5): 501-9[PubMed]
  • 5. Swarbrick MM, Havel PJ. Physiological, pharmacological, and nutritional regulation of circulating adiponectin concentrations in humans. Metab Syndr Relat Disord. 2008; 6(2): 87-102[DOI][PubMed]
  • 6. Havel PJ. Role of adipose tissue in body-weight regulation: mechanisms regulating leptin production and energy balance. Proc Nutr Soc. 2000; 59(3): 359-71[PubMed]
  • 7. Seufert J. Leptin effects on pancreatic beta-cell gene expression and function. Diabetes. 2004; 53 Suppl 1-8[PubMed]
  • 8. Kos K, Harte AL, da Silva NF, Tonchev A, Chaldakov G, James S, et al. Adiponectin and resistin in human cerebrospinal fluid and expression of adiponectin receptors in the human hypothalamus. J Clin Endocrinol Metab. 2007; 92(3): 1129-36[DOI][PubMed]
  • 9. Castellano JM, Navarro VM, Roa J, Pineda R, Sanchez-Garrido MA, Garcia-Galiano D, et al. Alterations in hypothalamic KiSS-1 system in experimental diabetes: early changes and functional consequences. Endocrinology. 2009; 150(2): 784-94[DOI][PubMed]
  • 10. Finn PD, Cunningham MJ, Pau KY, Spies HG, Clifton DK, Steiner RA. The stimulatory effect of leptin on the neuroendocrine reproductive axis of the monkey. Endocrinology. 1998; 139(11): 4652-62[DOI][PubMed]
  • 11. Dungan HM, Clifton DK, Steiner RA. Minireview: kisspeptin neurons as central processors in the regulation of gonadotropin-releasing hormone secretion. Endocrinology. 2006; 147(3): 1154-8[DOI][PubMed]
  • 12. Mead EJ, Maguire JJ, Kuc RE, Davenport AP. Kisspeptins: a multifunctional peptide system with a role in reproduction, cancer and the cardiovascular system. Br J Pharmacol. 2007; 151(8): 1143-53[DOI][PubMed]
  • 13. Castellano JM, Navarro VM, Fernandez-Fernandez R, Roa J, Vigo E, Pineda R, et al. Expression of hypothalamic KiSS-1 system and rescue of defective gonadotropic responses by kisspeptin in streptozotocin-induced diabetic male rats. Diabetes. 2006; 55(9): 2602-10[DOI][PubMed]
  • 14. Marroqui L, Gonzalez A, Neco P, Caballero-Garrido E, Vieira E, Ripoll C, et al. Role of leptin in the pancreatic beta-cell: effects and signaling pathways. J Mol Endocrinol. 2012; 49(1)-17[DOI][PubMed]
  • 15. Seufert J, Kieffer TJ, Leech CA, Holz GG, Moritz W, Ricordi C, et al. Leptin suppression of insulin secretion and gene expression in human pancreatic islets: implications for the development of adipogenic diabetes mellitus. J Clin Endocrinol Metab. 1999; 84(2): 670-6[DOI][PubMed]
  • 16. Caminos JE, Nogueiras R, Gaytan F, Pineda R, Gonzalez CR, Barreiro ML, et al. Novel expression and direct effects of adiponectin in the rat testis. Endocrinology. 2008; 149(7): 3390-402[DOI][PubMed]
  • 17. Cheng XB, Wen JP, Yang J, Yang Y, Ning G, Li XY. GnRH secretion is inhibited by adiponectin through activation of AMP-activated protein kinase and extracellular signal-regulated kinase. Endocrine. 2011; 39(1): 6-12[DOI][PubMed]
  • 18. Rodriguez-Pacheco F, Martinez-Fuentes AJ, Tovar S, Pinilla L, Tena-Sempere M, Dieguez C, et al. Regulation of pituitary cell function by adiponectin. Endocrinology. 2007; 148(1): 401-10[DOI][PubMed]
  • 19. Wen JP, Liu C, Bi WK, Hu YT, Chen Q, Huang H, et al. Adiponectin inhibits KISS1 gene transcription through AMPK and specificity protein-1 in the hypothalamic GT1-7 neurons. J Endocrinol. 2012; 214(2): 177-89[DOI][PubMed]
  • 20. Winzell MS, Nogueiras R, Dieguez C, Ahren B. Dual action of adiponectin on insulin secretion in insulin-resistant mice. Biochem Biophys Res Commun. 2004; 321(1): 154-60[DOI][PubMed]
  • 21. Pallett AL, Morton NM, Cawthorne MA, Emilsson V. Leptin inhibits insulin secretion and reduces insulin mRNA levels in rat isolated pancreatic islets. Biochem Biophys Res Commun. 1997; 238(1): 267-70[DOI][PubMed]
  • 22. Iwasa T, Matsuzaki T, Murakami M, Kinouchi R, Gereltsetseg G, Fujisawa S, et al. Sensitivities of mRNA expression levels of Kiss1 and its receptor, Kiss1r, to nutritional status are changed during the developmental period in female rats. J Endocrinol. 2010; 207(2): 195-202[DOI][PubMed]
  • 23. Castellano JM, Roa J, Luque RM, Dieguez C, Aguilar E, Pinilla L, et al. KiSS-1/kisspeptins and the metabolic control of reproduction: physiologic roles and putative physiopathological implications. Peptides. 2009; 30(1): 139-45[DOI][PubMed]
  • 24. Morelli A, Marini M, Mancina R, Luconi M, Vignozzi L, Fibbi B, et al. Sex steroids and leptin regulate the "first Kiss" (KiSS 1/G-protein-coupled receptor 54 system) in human gonadotropin-releasing-hormone-secreting neuroblasts. J Sex Med. 2008; 5(5): 1097-113[DOI][PubMed]
  • 25. Luque RM, Kineman RD, Tena-Sempere M. Regulation of hypothalamic expression of KiSS-1 and GPR54 genes by metabolic factors: analyses using mouse models and a cell line. Endocrinology. 2007; 148(10): 4601-11[DOI][PubMed]
  • 26. Qi Y, Takahashi N, Hileman SM, Patel HR, Berg AH, Pajvani UB, et al. Adiponectin acts in the brain to decrease body weight. Nat Med. 2004; 10(5): 524-9[DOI][PubMed]
  • 27. Wahab F, Bano R, Jabeen S, Irfan S, Shahab M. Effect of peripheral kisspeptin administration on adiponectin, leptin, and resistin secretion under fed and fasting conditions in the adult male rhesus monkey (Macaca mulatta). Horm Metab Res. 2010; 42(8): 570-4[DOI][PubMed]
  • 28. Staiger K, Stefan N, Staiger H, Brendel MD, Brandhorst D, Bretzel RG, et al. Adiponectin is functionally active in human islets but does not affect insulin secretory function or beta-cell lipoapoptosis. J Clin Endocrinol Metab. 2005; 90(12): 6707-13[DOI][PubMed]
  • 29. Gu W, Li X, Liu C, Yang J, Ye L, Tang J, et al. Globular adiponectin augments insulin secretion from pancreatic islet beta cells at high glucose concentrations. Endocrine. 2006; 30(2): 217-21[DOI][PubMed]
  • 30. Brown JE, Conner AC, Digby JE, Ward KL, Ramanjaneya M, Randeva HS, et al. Regulation of beta-cell viability and gene expression by distinct agonist fragments of adiponectin. Peptides. 2010; 31(5): 944-9[DOI][PubMed]
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