Oxidative stress and proteolytic activity in erythrocytes of diabetic patients

AUTHORS

Bolar Suryakanth Varashree 1 , * , Bhat Perumunda Gopalakrishna 2

1 Department of Biochemistry, Kasturba Medical College, Manipal University, [email protected], India

2 Department of Biochemistry, Kasturba Medical College, Manipal University, India

How to Cite: Varashree B, Gopalakrishna B. Oxidative stress and proteolytic activity in erythrocytes of diabetic patients, Int J Endocrinol Metab. Online ahead of Print ; 8(2):90-93.

ARTICLE INFORMATION

International Journal of Endocrinology and Metabolism: 8 (2); 90-93
Article Type: Original Article
Received: August 1, 2010
Accepted: January 26, 2011

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Abstract

Background: High blood glucose levels induce oxidative stress and decrease antioxidant defenses.
Objectives: The present study aims to assess the association, if any, between increase in proteolytic enzyme activities and oxidative stress in diabetic subjects.
Patients and Methods: Fifty-one non diabetic patients (33 men, 18 women, mean age 54.47 year) and 53 diabetic patients (39 men, 14 women, mean age 52.92year) were included in the study. Diabetic status was assessed by the fasting blood sugar (FBS) using glucose oxidase method. Oxidant stress was measured by estimating erythrocyte malondialdehyde (MDA) in terms of thiobarbituric acid reacting substance (TBARS). Proteolytic activity was estimated by the amount of amino groups released by the erythrocyte lysate of the diabetic individual using phenylhydrazine treated hemoglobin.
Results: Erythrocyte MDA was higher in diabetics (cases) (4.7±1.7 nmoles/gHb) than in controls (3.3±2.2 nmoles/gHb) (p=0.001). Erythrocyte proteolytic activity was also higher in cases (167.2±648 nmoles/gHb) than the controls (27.9±31.7 nmoles/gHb).
Conclusions: Both erythrocyte lipid peroxidation and the proteolytic activity in the erythrocyte lysates of diabetic patients was significantly higher in cases than in controls. Diabetes is associated with a significant increase in TBARS, an index of oxidant stress. Proteolytic enzymes degrade many oxidatively altered proteins preventing the accumulation of altered and damaged proteins in the cell.

 2011 Kowsar M.P.Co. All rights reserved.


Please cite this paper as:
Varashree BS, Gopalakrishna BP. Oxidative stress and proteolytic activity in erythrocytes of diabetic patients. Int J Endocrinol Metab. 2010;8(2):90-93.

Keywords

Erythrocyte Malondialdehyde Oxidative stress

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